SDS-PAGE Recipes


Resolving Gels
6% 5 mL 10 mL 15 mL 20 mL 25 mL 30 mL 40 mL 50 mL H2O 2.7 5.4 8.1 10.8 13.5 16.2 21.6 27.0 4x Resolving 1.25 2.5 3.75 5.0 6.25 7.5 10.0 12.5 30% Acryl/0.8%bis-Acryl 1.0 2.0 3.0 4.0 5.0 6.0 8.0 10.0 10% APS 0.050 0.100 0.150 0.200 0.250 0.300 0.400 0.500 TEMED 0.004 0.008 0.012 0.016 0.020 0.024 0.032 0.040 8% 5 mL 10 mL 15 mL 20 mL 25 mL 30 mL 40 mL 50 mL H2O 2.37 4.73 7.1 9.47 11.83 14.2 18.93 23.67 4x Resolving 1.25 2.5 3.75 5.0 6.25 7.5 10.0 12.5 30% Acryl/0.8%bis-Acryl 1.33 2.67 4.0 5.33 6.67 8.0 10.67 13.33 10% APS 0.050 0.100 0.150 0.200 0.250 0.300 0.400 0.500 TEMED 0.003 0.006 0.009 0.012 0.015 0.018 0.024 0.030 10% 5 mL 10 mL 15 mL 20 mL 25 mL 30 mL 40 mL 50 mL H2O 2.03 4.07 6.10 8.13 10.17 12.2 16.27 20.33 4x Resolving 1.25 2.5 3.75 5 6.25 7.5 10.0 12.5 30% Acryl/0.8%bis-Acryl 1.67 3.33 5.0 6.67 8.33 10.0 13.33 16.67 10% APS 0.050 0.100 0.150 0.200 0.250 0.300 0.400 0.500 TEMED 0.002 0.004 0.006 0.008 0.010 0.012 0.016 0.020 12% 5 mL 10 mL 15 mL 20 mL 25 mL 30 mL 40 mL 50 mL H2O 1.7 3.4 5.1 6.8 8.5 10.2 13.6 17.0 4x Resolving 1.25 2.5 3.75 5.0 6.25 7.5 10.0 12.5 30% Acryl/0.8%bis-Acryl 2.0 4.0 6.0 8.0 10.0 12.0 16.0 20.0 10% APS 0.050 0.100 0.150 0.200 0.250 0.300 0.400 0.500 TEMED 0.002 0.004 0.006 0.008 0.010 0.012 0.016 0.020 15% 5 mL 10 mL 15 mL 20 mL 25 mL 30 mL 40 mL 50 mL H2O 1.2 2.4 3.6 4.8 6 7.2 9.6 12.0 4x Resolving 1.25 2.5 3.75 5.0 6.25 7.5 10.0 12.5 30% Acryl/0.8%bis-Acryl 2.5 5 7.5 10.0 12.5 15.0 20.0 25.0 10% APS 0.050 0.100 0.150 0.200 0.250 0.300 0.400 0.500 TEMED 0.002 0.004 0.006 0.008 0.010 0.012 0.016 0.020
Stacking Gels
1 mL 2 mL 3 mL 4 mL 5 mL 6 mL 8 mL 10 mL H2O 0.573 1.147 1.72 2.29 2.87 3.44 4.59 5.73 4x Stacking 0.25 0.5 0.75 1.0 1.25 1.5 2.0 2.5 30% Acryl/0.8%bis-Acryl 0.167 0.333 0.5 0.667 0.833 1.0 1.33 1.67 10% APS 0.010 0.020 0.030 0.040 0.050 0.060 0.080 0.100 TEMED 0.001 0.002 0.003 0.004 0.005 0.006 0.008 0.010
Buffers
4x Resolving Buffer:
1.5 M Tris-Cl, pH 8.8
0.4% SDS
4x Stacking Buffer:
0.5 M Tris-Cl, pH 6.8
0.4% SDS
2x Sample Buffer:
125 mM Tris-Cl, pH 6.8
20% Glycerol
4% SDS
10% BME
0.04% Bromophenol Blue
0.4% SDS

6%	5 mL	10 mL	15 mL	20 mL	25 mL	30 mL	40 mL	50 mL
H2O	2.7	5.4	8.1	10.8	13.5	16.2	21.6	27.0
4x Resolving	1.25	2.5	3.75	5.0	6.25	7.5	10.0	12.5
30% Acryl/0.8%bis-Acryl	1.0	2.0	3.0	4.0	5.0	6.0	8.0	10.0
10% APS	0.050	0.100	0.150	0.200	0.250	0.300	0.400	0.500
TEMED	0.004	0.008	0.012	0.016	0.020	0.024	0.032	0.040

8%	5 mL	10 mL	15 mL	20 mL	25 mL	30 mL	40 mL	50 mL
H2O	2.37	4.73	7.1	9.47	11.83	14.2	18.93	23.67
4x Resolving	1.25	2.5	3.75	5.0	6.25	7.5	10.0	12.5
30% Acryl/0.8%bis-Acryl	1.33	2.67	4.0	5.33	6.67	8.0	10.67	13.33
10% APS	0.050	0.100	0.150	0.200	0.250	0.300	0.400	0.500
TEMED	0.003	0.006	0.009	0.012	0.015	0.018	0.024	0.030

10%	5 mL	10 mL	15 mL	20 mL	25 mL	30 mL	40 mL	50 mL
H2O	2.03	4.07	6.10	8.13	10.17	12.2	16.27	20.33
4x Resolving	1.25	2.5	3.75	5	6.25	7.5	10.0	12.5
30% Acryl/0.8%bis-Acryl	1.67	3.33	5.0	6.67	8.33	10.0	13.33	16.67
10% APS	0.050	0.100	0.150	0.200	0.250	0.300	0.400	0.500
TEMED	0.002	0.004	0.006	0.008	0.010	0.012	0.016	0.020

	1 mL	2 mL	3 mL	4 mL	5 mL	6 mL	8 mL	10 mL
H2O	0.573	1.147	1.72	2.29	2.87	3.44	4.59	5.73
4x Stacking	0.25	0.5	0.75	1.0	1.25	1.5	2.0	2.5
30% Acryl/0.8%bis-Acryl	0.167	0.333	0.5	0.667	0.833	1.0	1.33	1.67
10% APS	0.010	0.020	0.030	0.040	0.050	0.060	0.080	0.100
TEMED	0.001	0.002	0.003	0.004	0.005	0.006	0.008	0.010